In order to investigate on the frequency and antibody levels to Actinobacillus actinomycetemcomitans from Korean population, normal group were divided into 5 category by the related age : 3-6 years, 7-12 years, 13-20 years, 21-35 years and over 36 years, and also disease group were divided into the diagnostic condition : Localized juvenile periodontitis, post-localized juvenile periodontitis, rapidly progressive periodontitis, adult periodontitis.
Each group was composed of 10 to 20 patients and plaque sampling was also performed on the mesial pocket of mandibular Ist molar with 3 paper points on anaerobic condition. Isolation and identification were done by using a selective medium(tryptic soy agar supplemented with 10% serum, 75ug of Bacitracin and 5ug of Vancomycin per ml) in 10% CO2 incubator for 3 days with routine Gram staining, biochemical test and colony morphology.
Serum antibody levels were also performed with isolated A. actinomycetemcomitans SNUDC 10-1 by using modified ELISA technique.
The frequency of A. actinomycetemcomitans in normal healthy group was within 10% and no detection was revealed in 7 years group. Otherwise, the frequency of localized juvenile, rapidly progressive, adult periodontitis and adult periodontitis associated with diabetes mellitus were 7596, 68%, 4296 and 25%, respectively. Serum IgG antibody levels to A.a SNUDC 10-1 in umbrical cord blood was similar to 3-6 years or 13-20 years group, and 3-6 months group were shown only trace amount of antibody levels. The antibody levels of 1 year and 7-12 years group were revealed 2/41/3 levels of 3-6 years group and this levels were gradually increased from 7-12 years group until over 36 years group. However, the antibody levels of localized juvenile, post-localized juvenile and rapidly progressive periodontitis were significantly higher than that of healthy group and no significant differences of the level were recognized between each disease group.
Serum IgM antibody levels to A.a SNUDC 10-1 of umbrical cord group was only trace a-mount and this level was increased gradually with the increasing of age until 21-35 years group. Otherwise, after 36 years group showed low antibody level just like 1 year group However, the antibody levels of Localized juvenile, post-localized juvenile and rapidly progressive periodontitis were significantly increased to compare wih healthy control.
These results suggested that A. actinomycetemcomitans should be infected in infant age age and detection of specific antibody levels from the family of each patients could be clarify on the route of infection and diagnosis or prognosis of disease progression.
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